| Short Description : | Rabbit polyclonal PFDN1 (1-122) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PFDN1 |
| Gene ID: | 5201 |
| Uniprot ID: | PFD1_HUMAN |
| Immunogen Region: | 1-122 |
| Immunogen Sequence: | MAAPVDLELKKAFTELQAKV IDTQQKVKLADIQIEQLNRT KKHAHLTDTEIMTLVDETNM YEGVGRMFILQSKEAIHSQL LEKQKIAEEKIKELEQKKSY LERSVKEAEDNIREMLMARR AQ |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human PFDN1 (NP_002613.2). |
| Function | Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. |
| Protein Name | Prefoldin Subunit 1 |
| Database Links | Reactome: R-HSA-389957 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Prefoldin Subunit 1 antibodyAnti-PFDN1 antibodyAnti-PFD1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89680/358173/STJ11100742_1__83977.1713122480.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.")
