Anti-PF4 antibody [R09-7H9] (STJA0036515)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human PF4
STJA0036515
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General Information

Short DescriptionRabbit monoclonal anti-PF4 for use in WB, IHC-P and IP in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IP
HostRabbit
ReactivityHuman/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR09-7H9
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IHC 1:50-1:100
IP 1:50
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolPF4
Gene ID5196
Uniprot IDPLF4_HUMAN
ImmunogenA synthesized peptide derived from human PF4

Additional Info

Function Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity.
Protein Name Platelet Factor 4
Pf-4
C-X-C Motif Chemokine 4
Iroplact
Oncostatin-A Cleaved Into - Platelet Factor 4 - Short Form
Endothelial Cell Growth Inhibitor
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140875
Reactome: R-HSA-202733
Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-8936459
Cellular Localisation Secreted
Alternative Antibody Names Anti-Platelet Factor 4 antibody
Anti-Pf-4 antibody
Anti-C-X-C Motif Chemokine 4 antibody
Anti-Iroplact antibody
Anti-Oncostatin-A Cleaved Into - Platelet Factor 4 - Short Form antibody
Anti-Endothelial Cell Growth Inhibitor antibody
Anti-PF4 antibody
Anti-CXCL4 antibody
Anti-SCYB4 antibody

Information sourced from Uniprot.org

Citations

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