| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S9MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:4000 IHC-P:1:100-1:1000 IF/ICC:1:100-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PF4 |
| Gene ID: | 5196 |
| Uniprot ID: | PLF4_HUMAN |
| Immunogen Region: | 1-101 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-101 of human CXCL4/CXCL4/PF4 (P02776). |
| Immunogen Sequence: | MSSAAGFCASRPGLLFLGLL LLPLVVAFASAEAEEDGDLQ CLCVKTTSQVRPRHITSLEV IKAGPHCPTAQLIATLKNGR KICLDLQAPLYKKIIKKLLE S |
| Function | Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity. |
| Protein Name | Platelet Factor 4 Pf-4 C-X-C Motif Chemokine 4 Iroplact Oncostatin-A Cleaved Into - Platelet Factor 4 - Short Form Endothelial Cell Growth Inhibitor |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-140875 Reactome: R-HSA-202733 Reactome: R-HSA-380108 Reactome: R-HSA-418594 Reactome: R-HSA-8936459 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Platelet Factor 4 antibody Anti-Pf-4 antibody Anti-C-X-C Motif Chemokine 4 antibody Anti-Iroplact antibody Anti-Oncostatin-A Cleaved Into - Platelet Factor 4 - Short Form antibody Anti-Endothelial Cell Growth Inhibitor antibody Anti-PF4 antibody Anti-CXCL4 antibody Anti-SCYB4 antibody |
Information sourced from Uniprot.org