Host: |
Goat |
Applications: |
Pep-ELISA/WB/IF/FC |
Reactivity: |
Human/Mouse/Rat |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : |
Goat polyclonal antibody anti-Pericentrin 1/NUP85 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
Clonality : |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: |
0.5 mg/mL |
Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 75kDa band observed in lysates of cell line HeLa and approx. 70kDa in NIH3T3 cell lysates and in nuclear lysates of cell lines HeLa and HEK293 (calculated MW of 75.0kDa accordi |
Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: |
NUP85 |
Gene ID: |
79902 |
Uniprot ID: |
NUP85_HUMAN |
Immunogen Region: |
C-Term |
Accession Number: |
NP_079120.1 |
Specificity: |
Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and n |
Immunogen Sequence: |
ARAIIREGSLEGS |
Information sourced from Uniprot.org