Anti-PECAM1 antibody (686-735 aa) (STJ92117)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PECAM-1 at the amino acid range 686-735
General Information
| Short Description | Rabbit polyclonal anti-Platelet endothelial cell adhesion molecule (686-735 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:100-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PECAM1 |
| Gene ID | 5175 |
| Uniprot ID | PECA1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PECAM-1 at the amino acid range 686-735 |
| Immunogen Region | 686-735 aa |
| Specificity | CD31 Polyclonal Antibody detects endogenous levels of CD31 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
| Function | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. |
| Protein Name | Platelet Endothelial Cell Adhesion MoleculePecam-1EndocamGpiia'Peca1Cd Antigen Cd31 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-202733Reactome: R-HSA-210990Reactome: R-HSA-216083Reactome: R-HSA-432142Reactome: R-HSA-6798695Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated StimulationIsoform Long: Cell MembraneMembrane RaftCell JunctionLocalizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial CellsIsoform Delta15: Cell Junction |
| Alternative Antibody Names | Anti-Platelet Endothelial Cell Adhesion Molecule antibodyAnti-Pecam-1 antibodyAnti-Endocam antibodyAnti-Gpiia' antibodyAnti-Peca1 antibodyAnti-Cd Antigen Cd31 antibodyAnti-PECAM1 antibody |
Information sourced from Uniprot.org