• Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of HepG2 cells, using PDK1/PDHK1 antibody (STJ24945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PDK1/PDHK1 antibody (STJ24945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PDK1 antibody (337-436) (STJ24945)

SKU:
STJ24945

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDK1 (337-436) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDK1
Gene ID: 5163
Uniprot ID: PDK1_HUMAN
Immunogen Region: 337-436
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 337-436 of human PDK1/PDHK1 (NP_002601.1).
Immunogen Sequence: STAPRPRVETSRAVPLAGFG YGLPISRLYAQYFQGDLKLY SLEGYGTDAVIYIKALSTDS IERLPVYNKAAWKHYNTNHE ADDWCVPSREPKDMTTFRSA
Tissue Specificity Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
Post Translational Modifications Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Protein Name Pyruvate Dehydrogenase
Acetyl-Transferring Kinase Isozyme 1 - Mitochondrial
Pyruvate Dehydrogenase Kinase Isoform 1
Pdh Kinase 1
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase antibody
Anti-Acetyl-Transferring Kinase Isozyme 1 - Mitochondrial antibody
Anti-Pyruvate Dehydrogenase Kinase Isoform 1 antibody
Anti-Pdh Kinase 1 antibody
Anti-PDK1 antibody
Anti-PDHK1 antibody

Information sourced from Uniprot.org

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