• Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (dilution 1:100).
  • Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.

Anti-PDHA1 antibody [3H2-F8-B5] (STJ99056)

SKU:
STJ99056

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ICC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 3H2-F8-B5
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHA1
Gene ID: 5160
Uniprot ID: ODPA_HUMAN
Specificity: This antibody detects endogenous levels of pyruvate dehydrogenase (lipoamide) alpha 1 and does not cross-react with related proteins.
Immunogen: Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial
Pdhe1-A Type I
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibody
Anti-Pdhe1-A Type I antibody
Anti-PDHA1 antibody
Anti-PHE1A antibody

Information sourced from Uniprot.org

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