| Short Description : | Mouse monoclonal anti-Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial for use in WB and ICC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ICC |
| Host: | Mouse |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 3H2-F8-B5 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:1000ICC 1:100 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDHA1 |
| Gene ID: | 5160 |
| Uniprot ID: | ODPA_HUMAN |
| Immunogen: | Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli. |
| Specificity: | This antibody detects endogenous levels of pyruvate dehydrogenase (lipoamide) alpha 1 and does not cross-react with related proteins. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9837999Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibodyAnti-Pdhe1-A Type I antibodyAnti-PDHA1 antibodyAnti-PHE1A antibody |
Information sourced from Uniprot.org
