• STJ73600 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73600 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PDGFB precursor antibody (C-Term) (STJ73600)

SKU:
STJ73600-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow/Sheep
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PDGFB precursor (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
IF: Strong expression of the protein seen in the vesicles of MCF7 cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/m
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PDGFB
Gene ID: 5155
Uniprot ID: PDGFB_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002599.1; NP_148937.1
Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_002599.1; NP_148937.1).
Immunogen Sequence: KHRKFKHTHDKT
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
Protein Name Platelet-Derived Growth Factor Subunit B
Pdgf Subunit B
Pdgf-2
Platelet-Derived Growth Factor B Chain
Platelet-Derived Growth Factor Beta Polypeptide
Proto-Oncogene C-Sis
Becaplermin
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1257604
Reactome: R-HSA-186763
Reactome: R-HSA-186797
Reactome: R-HSA-2219530
Reactome: R-HSA-3000171
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Cellular Localisation Secreted
Released By Platelets Upon Wounding
Alternative Antibody Names Anti-Platelet-Derived Growth Factor Subunit B antibody
Anti-Pdgf Subunit B antibody
Anti-Pdgf-2 antibody
Anti-Platelet-Derived Growth Factor B Chain antibody
Anti-Platelet-Derived Growth Factor Beta Polypeptide antibody
Anti-Proto-Oncogene C-Sis antibody
Anti-Becaplermin antibody
Anti-PDGFB antibody
Anti-PDGF2 antibody
Anti-SIS antibody

Information sourced from Uniprot.org

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