• Western blot analysis of extracts of rat brain, using PDE10A antibody (STJ118046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of HeLa cells using PDE10A rabbit polyclonal antibody (STJ118046) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDE10A antibody (23-200) (STJ118046)

SKU:
STJ118046

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE10A (23-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE10A
Gene ID: 10846
Uniprot ID: PDE10_HUMAN
Immunogen Region: 23-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-200 of human PDE10A (NP_001124162.1).
Immunogen Sequence: SLTDEKVKAYLSLHPQVLDE FVSESVSAETVEKWLKRKNN KSEDESAPKEVSRYQDTNMQ GVVYELNSYIEQRLDTGGDN QLLLYELSSIIKIATKADGF ALYFLGECNNSLCIFTPPGI KEGKPRLIPAGPITQGTTVS AYVAKSRKTLLVEDILGDER FPRGTGLESGTRIQSVLC
Tissue Specificity Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
Post Translational Modifications Isoform PDE10A2: Phosphorylated on Thr-16.
Function Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.
Protein Name Camp And Camp-Inhibited Cgmp 3' -5'-Cyclic Phosphodiesterase 10a
Database Links Reactome: R-HSA-418457
Reactome: R-HSA-418555
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Camp And Camp-Inhibited Cgmp 3' -5'-Cyclic Phosphodiesterase 10a antibody
Anti-PDE10A antibody

Information sourced from Uniprot.org

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