• Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PCSK9 antibody (STJA0010204)

SKU:
STJA0010204

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Proprotein convertase subtilisin/kexin type 9 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCSK9
Gene ID: 255738
Uniprot ID: PCSK9_HUMAN
Specificity: This antibody detects endogenous levels of Human PCSK9
Immunogen: Synthesized peptide derived from human PCSK9
Post Translational Modifications Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis.
Function Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Protein Name Proprotein Convertase Subtilisin/Kexin Type 9
Neural Apoptosis-Regulated Convertase 1
Narc-1
Proprotein Convertase 9
Pc9
Subtilisin/Kexin-Like Protease Pc9
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8866427
Reactome: R-HSA-8957275
Reactome: R-HSA-8964038
Cellular Localisation Cytoplasm
Secreted
Endosome
Lysosome
Cell Surface
Endoplasmic Reticulum
Golgi Apparatus
Autocatalytic Cleavage Is Required To Transport It From The Endoplasmic Reticulum To The Golgi Apparatus And For The Secretion Of The Mature Protein
Localizes To The Endoplasmic Reticulum In The Absence Of Ldlr And Colocalizes To The Cell Surface And To The Endosomes/Lysosomes In The Presence Of Ldlr
The Sorting To The Cell Surface And Endosomes Is Required In Order To Fully Promote Ldlr Degradation
Alternative Antibody Names Anti-Proprotein Convertase Subtilisin/Kexin Type 9 antibody
Anti-Neural Apoptosis-Regulated Convertase 1 antibody
Anti-Narc-1 antibody
Anti-Proprotein Convertase 9 antibody
Anti-Pc9 antibody
Anti-Subtilisin/Kexin-Like Protease Pc9 antibody
Anti-PCSK9 antibody
Anti-NARC1 antibody
Anti-PSEC0052 antibody

Information sourced from Uniprot.org

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