| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-PCQAP/MED15 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 90kDa band observed in Human Lung lysates (calculated MW of 86.8kDa according to NP_001003891.1). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
MED15 |
| Gene ID: |
51586 |
| Uniprot ID: |
MED15_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001003891.1; NP_056973.2 |
| Specificity: |
This antibody is expected to recognize both reported isoforms (NP_001003891.1; NP_056973.2). |
| Immunogen Sequence: |
PPQSQTQPLVSQAQ |
| Post Translational Modifications | Ubiquitinated by TRIM11, leading to proteasomal degradation. |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 15Activator-Recruited Cofactor 105 Kda ComponentArc105Ctg Repeat Protein 7aMediator Complex Subunit 15Positive Cofactor 2 Glutamine/Q-Rich-Associated ProteinPc2 Glutamine/Q-Rich-Associated ProteinTpa-Inducible Gene 1 ProteinTig-1Trinucleotide Repeat-Containing Gene 7 Protein |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-212436Reactome: R-HSA-381340Reactome: R-HSA-9833110 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 15 antibodyAnti-Activator-Recruited Cofactor 105 Kda Component antibodyAnti-Arc105 antibodyAnti-Ctg Repeat Protein 7a antibodyAnti-Mediator Complex Subunit 15 antibodyAnti-Positive Cofactor 2 Glutamine/Q-Rich-Associated Protein antibodyAnti-Pc2 Glutamine/Q-Rich-Associated Protein antibodyAnti-Tpa-Inducible Gene 1 Protein antibodyAnti-Tig-1 antibodyAnti-Trinucleotide Repeat-Containing Gene 7 Protein antibodyAnti-MED15 antibodyAnti-ARC105 antibodyAnti-CTG7A antibodyAnti-PCQAP antibodyAnti-TIG1 antibodyAnti-TNRC7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
