Anti-PCNA antibody [SM4907] (STJA0004907)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse PCNA
General Information
| Short Description | Mouse monoclonal anti-PCNA for use in WB, IHC, IF and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SM4907 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB (H, M, R) 1:500-1:1000IHC/IF (H, M, R) 1:1000-1:4000ICC/IF (H, M, R) 1:100-1:500 |
| Formulation | PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | Pcna |
| Gene ID | 18538 |
| Uniprot ID | PCNA_MOUSE |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
Additional Info
| Function | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Protein Name | Proliferating Cell Nuclear AntigenPcnaCyclin |
| Database Links | Reactome: R-MMU-110312Reactome: -MMU-110314Reactome: -MMU-110320Reactome: -MMU-174411Reactome: -MMU-174414Reactome: -MMU-174417Reactome: -MMU-174437Reactome: -MMU-4615885Reactome: -MMU-5358565Reactome: -MMU-5651801Reactome: -MMU-5655862Reactome: -MMU-5656121Reactome: -MMU-5656169Reactome: -MMU-5685942Reactome: -MMU-5696397Reactome: -MMU-5696400Reactome: -MMU-6782135Reactome: -MMU-6782210Reactome: -MMU-6804114Reactome: -MMU-69091Reactome: -MMU-69166Reactome: -MMU-69183Reactome: -MMU-8866654 |
| Cellular Localisation | NucleusColocalizes With CrebbpEp300 And Pold1 To Sites Of Dna DamageForms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S PhaseTogether With Apex2Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents |
| Alternative Antibody Names | Anti-Proliferating Cell Nuclear Antigen antibodyAnti-Pcna antibodyAnti-Cyclin antibodyAnti-Pcna antibody |
Information sourced from Uniprot.org