| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PCDH15 (160-400) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PCDH15 |
| Gene ID: |
65217 |
| Uniprot ID: |
PCD15_HUMAN |
| Immunogen Region: |
160-400 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human PCDH15 (NP_001136235.1). |
| Immunogen Sequence: |
TVNELTPVGTTIFTGFSGDN GATDIDDGPNGQIEYVIQYN PDDPTSNDTFEIPLMLTGNI VLRKRLNYEDKTRYFVIIQA NDRAQNLNERRTTTTTLTVD VLDGDDLGPMFLPCVLVPNT RDCRPLTYQAAIPELRTPEE LNPIIVTPPIQAIDQDRNIQ PPSDRPGILYSILVGTPEDY PRFFHMHPRTAELSLLEPVN RDFHQKFDLVIKAEQDNGHP LPAFAGLHIEILDENNQSP |
| Tissue Specificity | Expressed in brain, lung, kidney, spleen and testis. Found also in the inner and outer synaptic layers, and the nerve fiber layer in adult and fetal retinas. Found in the supporting cells, outer sulcus cells and spiral ganglion of fetal cochlea. Expressed in cytotoxic tumor-derived T- and NK-cell lines as well as biopsies of nasal NK/T-cell lymphomas. Not detected in normal or in vitro activated peripheral blood cells, CD4 or CD8 lymphocytes or NK cells. Isoform 3 is expressed in brain, heart, cerebellum and kidney. CD1 isoforms, such as isoform 1, have a limited pattern of expression and is detected in testis, retina and cochlea. CD2 isoforms, such as isoforms 4 and 5, are expressed in heart, kidney, thymus, spleen, testis, retina and cochlea. CD3 isoforms, such as isoform 6, are widely expressed. |
| Function | Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function. |
| Protein Name | Protocadherin-15 |
| Database Links | Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEfficient Localization To The Plasma Membrane Requires The Presence Of Lhfpl5Isoform 3: Secreted |
| Alternative Antibody Names | Anti-Protocadherin-15 antibodyAnti-PCDH15 antibodyAnti-USH1F antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NRD1 knockout (KO) 293T cells, using [KO Validated] NRD1 Rabbit polyclonal antibody (STJ11100733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89671/358166/STJ11100733_1__46368.1713122470.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NRD1 knockout (KO) 293T cells, using [KO Validated] NRD1 Rabbit polyclonal antibody (STJ11100733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
