| Host: | Rabbit |
| Applications: | WB/IHC-P/IP |
| Reactivity: | Human/Rat/Hamster |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Paxillin for use in WB, IHC-P and IP in Human, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R07-1B4 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IP 1:20 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | PXN |
| Gene ID: | 5829 |
| Uniprot ID: | PAXI_HUMAN |
| Immunogen: | A synthetic peptide of human Paxillin |
| Post Translational Modifications | Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility. Phosphorylation at Ser-272 promotes focal adhesion disassembly during cell migration. |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion. |
| Protein Name | Paxillin |
| Database Links | Reactome: R-HSA-180292Reactome: R-HSA-4420097Reactome: R-HSA-445355Reactome: R-HSA-446343Reactome: R-HSA-446388Reactome: R-HSA-8849471Reactome: R-HSA-9926550 |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionFocal AdhesionCell CortexColocalizes With Integrins At The Cell PeripheryColocalize With Pxn To Membrane Ruffles And The Leading Edge Of Migrating Cells |
| Alternative Antibody Names | Anti-Paxillin antibodyAnti-PXN antibody |
Information sourced from Uniprot.org