| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Paxillin (C-Term) for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000 |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | PXN |
| Gene ID: | 5829 |
| Uniprot ID: | PAXI_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells. |
| Immunogen: | A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Paxillin. This peptide sequence is highly conserved in rat and mouse paxillins, and is found in all three paxillin isoforms ( Alpha, Beta, and |
| Immunogen Sequence: | Human |
| Post Translational Modifications | Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility. Phosphorylation at Ser-272 promotes focal adhesion disassembly during cell migration. |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion. |
| Protein Name | Paxillin |
| Database Links | Reactome: R-HSA-180292Reactome: R-HSA-4420097Reactome: R-HSA-445355Reactome: R-HSA-446343Reactome: R-HSA-446388Reactome: R-HSA-8849471Reactome: R-HSA-9926550 |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionFocal AdhesionCell CortexColocalizes With Integrins At The Cell PeripheryColocalize With Pxn To Membrane Ruffles And The Leading Edge Of Migrating Cells |
| Alternative Antibody Names | Anti-Paxillin antibodyAnti-PXN antibody |
Information sourced from Uniprot.org
