Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PAX1 using PAX1 Rabbit polyclonal antibody (STJ119605) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PAX1 antibody (313-328) (STJ119605)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119605
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General Information

Short DescriptionRabbit polyclonal PAX1 (313-328) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPAX1
Gene ID5075
Uniprot IDPAX1_HUMAN
Immunogen Region313-328
Immunogen SequenceTFMEQTGALAGSEGTAYSPK MEDWAGVNRTAFPATPAVNG LEKPALEADIKYTQSASTLS AVGGFLPACAYPASNQHGVY SAPGGGYLAPGPPWPPAQGP PLAPPGAGVAVHGGELAAAM TFKHPSREGSLPAPAARPRT PSVAYTDCPSRPRPPRGSSP RTRARRERQADPGAQVCAAA PAIGTGRIGGLAEEEASAGP RGARPASPQAQPCLWPDPPH FLYWSGFLGFSELGF
SpecificityA synthetic peptide corresponding to a sequence within amino acids 300-400 of human PAX1 (NP_006183.2).

Additional Info

Function This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column.
Protein Name Paired Box Protein Pax-1
Hup48
Database Links
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Paired Box Protein Pax-1 antibody
Anti-Hup48 antibody
Anti-PAX1 antibody
Anti-HUP48 antibody

Information sourced from Uniprot.org

Citations

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