• Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine polyclonal antibody (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Blue: DAPI for nuclear staining. C6 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. C6 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine polyclonal antibody (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody (STJ11101040). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (STJ11101040) at a dilition of 1:1000. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes.

Anti-Pan-Phospho-Tyrosine antibody (STJ11101040)

SKU:
STJ11101040

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
Background Tyrosine phosphorylation (pTyr) , much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.

Information sourced from Uniprot.org

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