• Western blot analysis of extracts of various cell lines, using Pan Phospho-Tyrosine antibody (STJ11102650) at 1:1000 dilution. Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat (pervanadate) cells using 3 Mu g Pan Phospho-Tyrosine antibody (STJ11102650). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (STJ11102650) at a dilution of 1:1000.

Anti-Pan-Phospho-Tyrosine antibody [S0MR] (STJ11102650)

SKU:
STJ11102650

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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A random peptide library phosphorylated peptide around Tyr.
Background Tyrosine phosphorylation (pTyr) , much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance