• Western blot analysis of extracts of various cell lines, using Pan Phospho-Tyrosine antibody (STJ11102650) at 1:1000 dilution. Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat (pervanadate) cells using 3 Mu g Pan Phospho-Tyrosine antibody (STJ11102650). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (STJ11102650) at a dilution of 1:1000.

Anti-Pan-Phospho-Tyrosine antibody [S0MR] (STJ11102650)

SKU:
STJ11102650

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Pan-Phospho-Tyrosine is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A random peptide library phosphorylated peptide around Tyr.
Immunogen Sequence: Email for sequence
Background This gene encodes a protein that is a component of the BLOC1 multi-subunit protein complex. This complex is necessary for the biogenesis of specialized organelles of the endosomal-lysosomal system, including platelet dense granules and melanosomes. Mutations in this gene cause Hermansky-Pudlak syndrome 8, a disease characterized by lysosomal storage defects, bleeding due to platelet storage pool deficiency, and oculocutaneous albinism.

Information sourced from Uniprot.org

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