• Western blot analysis of extracts of Jurkat cells, using pan Phospho-Serine/Threonine antibody (STJ11101105) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from Jurkat cells, using pan Phospho-Serine/Threonine Mouse monoclonal antibody (STJ11101105) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Phospho-Serine/Threonine antibody [S5MM] (STJ11101105)

SKU:
STJ11101105

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Host: Mouse
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MM
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to a sequence containing phosphorylated Serine/Threonine.
Background Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Global alteration of signal transduction by inhibition of serine/threonine dephosphorylation has recently been shown to markedly potentiate cancer cell killing by the DNA-methylating drug, temozolomide.

Information sourced from Uniprot.org

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