• Dot-blot analysis of all sorts using pan-methyl-Histidine polyclonal antibody (STJ11103884) at 1:1000 dilution. Peptide 1：Peptide library (3-methylhistidine-intermediate) Peptide 2：Peptide library (3-methylhistidine-C-terminal) Peptide 3：Peptide library (1-methylhistidine-intermediate) Peptide 4：Peptide library (1-methylhistidine-C-terminal) Peptide 5：Histidine methylation modified peptide library mixPeptide 6：Control peptide library mix
  • Western blot analysis of extracts of various cell lines, using pan-methyl-Histidine antibody (STJ11103884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-pan-Methyl-Histidine antibody (STJ11103884)

SKU:
STJ11103884

£129.50 - £642.50
Current Stock:
Host: Rabbit
Applications: WB/DB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
DB:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide of Methylated Histidine His.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance