• Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ11100252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Pan-Di-Methyl-Kme2 antibody (STJ11100252)

SKU:
STJ11100252

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to a sequence containing dimethylated K.
Background The lysine methylation chemical reaction is the reversible addition of one, two or three methyl groups to the Epsilon-nitrogen of a lysine side chain, forming monomethylated, dimethylated and trimethylated derivatives (referred to here as ‘Kme1 NA, ‘Kme2 and ‘Kme3 ).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance