Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit. CK-pan mouse monoclonal antibody (STJ197095) green, Ki-67 rabbit monoclonal antibody (STJA0006295 red), Iba 1 mouse monoclonal antibody (purple), CD8 a mouse monoclonal antibody (cyan), CD20 mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-Pan-Cytokeratin antibody (100-200 aa) [ABT154] (STJ197095)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenSynthesized peptide derived from the human Cytokeratin Pan at the amino acid range 100-200
STJ197095
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General Information

Short DescriptionMouse monoclonal anti-Pan-Cytokeratin (100-200 aa) for use in IHC, WB and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB/IF
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDABT154
IsotypeIgG1k
ConjugationUnconjugated
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution RangeIHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCDH2
CDH4
1000
1002
1014
CADH2_HUMAN
CADH4_HUMAN
CAD16_HUMAN
ImmunogenSynthesized peptide derived from the human Cytokeratin Pan at the amino acid range 100-200
Immunogen Region100-200 aa
SpecificityThe antibody can recognize multiple human Cytokeratins, including CK10, 13, 14, 15, 16, 18, 19, and it can be used for immunohistochemical detection of tumors from monolayer and multilayered epithel

Citations

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