• Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit. CK-pan mouse monoclonal antibody (STJ197095) green, Ki-67 rabbit monoclonal antibody (STJA0006295 red), Iba 1 mouse monoclonal antibody (purple), CD8 a mouse monoclonal antibody (cyan), CD20 mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).
  • HCT116whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK20 (ABT006) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HCT116Predicted band size: 50-60kDaObserved band size: 52, 55kDa
  • Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin Pan (ABT154) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 50-60kDaObserved band size: 52kDa
  • Human endometrial adenocarcinoma tissue was stained with Anti-Cytokeratin (ABT154) Antibody
  • Human esophageal squamous cell carcinoma tissue was stained with Anti-Cytokeratin (ABT154) Antibody
  • Human hepatocellular carcinoma tissue was stained with Anti-Cytokeratin (ABT154) Antibody
  • Human lung adenocarcinoma tissue was stained with Anti-Cytokeratin (ABT154) Antibody
  • Human prostate tissue was stained with Anti-Cytokeratin (ABT154) Antibody
  • Fluorescence multiplex immunohistochemical analysis of normal human appendix tissue (formalin-fixed paraffin-embedded section). The section was incubated in 3 rounds of staining; in the order of CK PAN. (STJ197095 1/200 dilution), PD-1. (STJ196857 1/200 dilution), Caldesmon pan. (STJ197106 1/200 dilution), each using a separate fluorescent tyramide signal amplification system : Treble-Fluorescence immunohistochemical mouse/rabbit kit (pH9.0)

Anti-Pan-Cytokeratin antibody (100-200 aa) [ABT154] (STJ197095)

SKU:
STJ197095

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Pan-Cytokeratin (100-200 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT154
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH4
CDH1
1002
999
1001
CADH4_HUMAN
CADH1_HUMAN
CADH3_HUMAN
Immunogen Region: 100-200 aa
Specificity: The antibody can recognize multiple human Cytokeratins, including CK10, 13, 14, 15, 16, 18, 19, and it can be used for immunohistochemical detection of tumors from monolayer and multilayered epithel
Immunogen: Synthesized peptide derived from the human Cytokeratin Pan at the amino acid range 100-200
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance