• STJ70617 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70617 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70617 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Anti-PAD4/PADI4 antibody (N-Term) (STJ70617)

SKU:
STJ70617-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PAD4/PADI4 (N-Term) is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nucleus of HeLa and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PADI4
Gene ID: 23569
Uniprot ID: PADI4_HUMAN
Immunogen Region: N-Term
Accession Number: NP_036519.2
Immunogen Sequence: AQGTLIRVTPEQPC
Post Translational Modifications Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs).NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Protein Name Protein-Arginine Deiminase Type-4
Hl-60 Pad
Peptidylarginine Deiminase Iv
Protein-Arginine Deiminase Type Iv
Database Links Reactome: R-HSA-3247509
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Granule
Cytoplasmic Granules Of Eosinophils And Neutrophils
Alternative Antibody Names Anti-Protein-Arginine Deiminase Type-4 antibody
Anti-Hl-60 Pad antibody
Anti-Peptidylarginine Deiminase Iv antibody
Anti-Protein-Arginine Deiminase Type Iv antibody
Anti-PADI4 antibody
Anti-PAD4 antibody
Anti-PADI5 antibody
Anti-PDI5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance