| Host: |
Mouse |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-p21 is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
DCS-60.2 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide |
| Dilution Range: |
IHC 1:50 – 1:200 |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Tissue Specificity | Expressed in all adult tissues, with 5-fold lower levels observed in the brain. |
| Post Translational Modifications | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
| Function | Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1Cdk-Interacting Protein 1Melanoma Differentiation-Associated Protein 6Mda-6P21 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-198323Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-5674400Reactome: R-HSA-6785807Reactome: R-HSA-6804116Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-69656Reactome: R-HSA-69895Reactome: R-HSA-8852276Reactome: R-HSA-8866911Reactome: R-HSA-8878166Reactome: R-HSA-8941855Reactome: R-HSA-8951664Reactome: R-HSA-9616222Reactome: R-HSA-9617828Reactome: R-HSA-9661069Reactome: R-HSA-9702518Reactome: R-HSA-9703465Reactome: R-HSA-9755511 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 1 antibodyAnti-Cdk-Interacting Protein 1 antibodyAnti-Melanoma Differentiation-Associated Protein 6 antibodyAnti-Mda-6 antibodyAnti-P21 antibodyAnti-CDKN1A antibodyAnti-CAP20 antibodyAnti-CDKN1 antibodyAnti-CIP1 antibodyAnti-MDA6 antibodyAnti-PIC1 antibodyAnti-SDI1 antibodyAnti-WAF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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