• Western blot analysis of extracts of various cell lines, using OTX2 Rabbit monoclonal antibody (STJ11102051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using OTX2 Rabbit monoclonal antibody (STJ11102051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-OTX2 antibody (190-289) [S1MR] (STJ11102051)

SKU:
STJ11102051

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OTX2
Gene ID: 5015
Uniprot ID: OTX2_HUMAN
Immunogen Region: 190-289
Specificity: A synthetic peptide corresponding to a sequence within amino acids 190-289 of human OTX2 (P32243).
Immunogen Sequence: AGSTSYFGGMDCGSYLTPMH HQLPGPGATLSPMGTNAVTS HLNQSPASLSTQGYGASSLG FNSTTDCLDYKDQTASWKLN FNADCLDYKDQTSSWKFQVL
Function Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Protein Name Homeobox Protein Otx2
Orthodenticle Homolog 2
Database Links Reactome: R-HSA-9823739
Reactome: R-HSA-9832991
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Otx2 antibody
Anti-Orthodenticle Homolog 2 antibody
Anti-OTX2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance