Anti-OTULIN antibody (284-334 aa) (STJ193477)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human F105B at the amino acid range 284-334
General Information
| Short Description | Rabbit polyclonal anti-Ubiquitin thioesterase otulin (284-334 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OTULIN |
| Gene ID | 90268 |
| Uniprot ID | OTUL_HUMAN |
| Immunogen | Synthesized peptide derived from the human F105B at the amino acid range 284-334 |
| Immunogen Region | 284-334 aa |
| Specificity | This antibody detects endogenous levels of F105B at Human/Mouse |
Additional Info
| Function | Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling. |
| Protein Name | Ubiquitin Thioesterase OtulinDeubiquitinating Enzyme OtulinOtu Domain-Containing Deubiquitinase With Linear Linkage SpecificityUbiquitin Thioesterase Gumby |
| Database Links | Reactome: R-HSA-5357905Reactome: R-HSA-8866652 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Thioesterase Otulin antibodyAnti-Deubiquitinating Enzyme Otulin antibodyAnti-Otu Domain-Containing Deubiquitinase With Linear Linkage Specificity antibodyAnti-Ubiquitin Thioesterase Gumby antibodyAnti-OTULIN antibodyAnti-FAM105B antibody |
Information sourced from Uniprot.org