Anti-Osteopontin/SPP1 antibody (C-Term) (STJ70731)

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STJ70731-100
€390.63
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-Osteopontin/SPP1 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 35kDa band observed in lysates of cell line MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1).
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SPP1
Gene ID: 6696
Uniprot ID: OSTP_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001035147.1; NP_000573.1; NP_001035149.1
Immunogen Sequence: DPKSKEEDKHLK
Post Translational Modifications Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Function Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Protein Name Osteopontin
Bone Sialoprotein 1
Nephropontin
Secreted Phosphoprotein 1
Spp-1
Urinary Stone Protein
Uropontin
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-186797
Reactome: R-HSA-216083
Reactome: R-HSA-381426
Reactome: R-HSA-8949275
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Osteopontin antibody
Anti-Bone Sialoprotein 1 antibody
Anti-Nephropontin antibody
Anti-Secreted Phosphoprotein 1 antibody
Anti-Spp-1 antibody
Anti-Urinary Stone Protein antibody
Anti-Uropontin antibody
Anti-SPP1 antibody
Anti-BNSP antibody
Anti-OPN antibody
Anti-PSEC0156 antibody

Information sourced from Uniprot.org