• Western blot analysis of extracts of various cell lines, using Oncostatin M rabbit monoclonal antibody (STJ11102360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of paraffin-embedded mouse spleen using Oncostatin M Rabbit monoclonal antibody (STJ11102360) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using Oncostatin M rabbit monoclonal antibody (STJ11102360) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat spleen using Oncostatin M Rabbit monoclonal antibody (STJ11102360) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using Oncostatin M rabbit monoclonal antibody (STJ11102360) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using Oncostatin M Rabbit monoclonal antibody (STJ11102360) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen using Oncostatin M rabbit monoclonal antibody (STJ11102360) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Oncostatin M Rabbit monoclonal antibody (STJ11102360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-OSM antibody (1-100) [S0MR] (STJ11102360)

SKU:
STJ11102360

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OSM
Gene ID: 5008
Uniprot ID: ONCM_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Oncostatin M (P13725).
Immunogen Sequence: MGVLLTQRTLLSLVLALLFP SMASMAAIGSCSKEYRVLLG QLQKQTDLMQDTSRLLDPYI RIQGLDVPKLREHCRERPGA FPSEETLRGLGRRGFLQTLN
Post Translational Modifications Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.
Function Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Protein Name Oncostatin-M
Osm
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-6788467
Cellular Localisation Secreted
Alternative Antibody Names Anti-Oncostatin-M antibody
Anti-Osm antibody
Anti-OSM antibody

Information sourced from Uniprot.org

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