| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | O.sativa |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 82-276 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 82-276 of Oryza sativa OsBZR1 (Q7XI96). |
| Immunogen Sequence: | PSSAGGASVGMSPCSSTQLL SAPSSSFPSPVPSYHASPAS SSFPSPSRIDNPSASCLLPF LRGLPNLPPLRVSSSAPVTP PLSSPTASRPPKIRKPDWDV DPFRHPFFAVSAPASPTRGR RLEHPDTIPECDESDVSTVD SGRWISFQMATTAPTSPTYN LVNPGASTSNSMEIEGTAGR GGAEFEFDKGRVTPW |
| Background | Positive brassinosteroid-signaling protein. Mediates downstream brassinosteroid-regulated growth response and feedback inhibition of brassinosteroid (BR) biosynthetic genes. May act as transcriptional repressor by binding the brassinosteroid-response element (BREE) (5 NA-CGTG (T/C) G-3 ) in the promoter of DLT (AC Q9LWU9) , another positive regulator of BR signaling. Acts as transcriptional repressor of LIC, a negative regulator of BR signaling, by binding to the BRRE element of its promoter. BZR1 and LIC play opposite roles in BR signaling and regulation of leaf bending |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and BCKDHA knockout (KO) 293T cells, using [KO Validated] BCKDHA Rabbit pAb (STJ11106587) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/185783/407691/STJ11106587_1__02232.1744894218.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BCKDHA knockout (KO) 293T cells, using [KO Validated] BCKDHA Rabbit pAb (STJ11106587) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
