• Western blot analysis of various lysates using ORC1 Rabbit polyclonal antibody (STJ116956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ORC1 antibody (696-856) (STJ116956)

SKU:
STJ116956

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ORC1 (696-856) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ORC1
Gene ID: 4998
Uniprot ID: ORC1_HUMAN
Immunogen Region: 696-856
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 696-856 of human ORC1 (NP_001177748.1).
Immunogen Sequence: EDDAIQLVARKVAALSGDAR RCLDICRRATEICEFSQQKP DSPGLVTIAHSMEAVDEMFS SSYITAIKNSSVLEQSFLRA ILAEFRRSGLEEATFQQIYS QHVALCRMEGLPYPTMSETM AVCSHLGSCRLLLVEPSRND LLLRVRLNVSQDDVLYALKD E
Post Translational Modifications Phosphorylated during mitosis.
Function Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Protein Name Origin Recognition Complex Subunit 1
Replication Control Protein 1
Database Links Reactome: R-HSA-113507
Reactome: R-HSA-176187
Reactome: R-HSA-68616
Reactome: R-HSA-68689
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69205
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Origin Recognition Complex Subunit 1 antibody
Anti-Replication Control Protein 1 antibody
Anti-ORC1 antibody
Anti-ORC1L antibody
Anti-PARC1 antibody

Information sourced from Uniprot.org

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