| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ORAI1 (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ORAI1 |
| Gene ID: |
84876 |
| Uniprot ID: |
CRCM1_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ORAI1 (NP_116179.2). |
| Immunogen Sequence: |
MHPEPAPPPSRSSPELPPSG GSTTSGSRRSRRRSGDGEPP GAPPPPPSAVTYPDWIGQSY SEVMSLNEHSMQALSWRKLY LSRAKLKASSRTSALLSGFA |
| Tissue Specificity | Expressed in naive CD4 and CD8 T cells (at protein level). Expressed at similar levels in naive and effector T helper cells. |
| Post Translational Modifications | N-glycosylated. N-glycosylation inhibits channel activity in T cells. Ubiquitinated. Cys-195 is oxidated, leading to inactivation of channel activity. |
| Function | Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Plays a prominent role in Ca(2+) influx at the basolateral membrane of mammary epithelial cells independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca(2+) for milk secretion. |
| Protein Name | Calcium Release-Activated Calcium Channel Protein 1Protein Orai-1Transmembrane Protein 142a |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-5578775Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneIsoform Beta Is More Mobile In The Plasma MembraneColocalizes With Stim1 At The Cell Membrane |
| Alternative Antibody Names | Anti-Calcium Release-Activated Calcium Channel Protein 1 antibodyAnti-Protein Orai-1 antibodyAnti-Transmembrane Protein 142a antibodyAnti-ORAI1 antibodyAnti-CRACM1 antibodyAnti-TMEM142A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
