Anti-OCT4 antibody (1-135 aa) (STJ11107036)

SKU:
STJ11107036
$151.88 - $708.08
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Gene Symbol: POU5F1
Gene ID: 5460
Uniprot ID: PO5F1_HUMAN
Immunogen Region: 1-135 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human OCT4 (NP_002692.2).
Immunogen Sequence: MAGHLASDFAFSPPPGGGGD GPGGPEPGWVDPRTWLSFQG PPGGPGIGPGVGPGSEVWGI PPCPPPYEFCGGMAYCGPQV GVGLVPQGGLETSQPEGEAG VGVESNSDGASPEPCTVTPG AVKLEKEKLEQNPEE
Tissue Specificity Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Post Translational Modifications Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Protein Name Pou Domain - Class 5 - Transcription Factor 1
Octamer-Binding Protein 3
Oct-3
Octamer-Binding Protein 4
Oct-4
Octamer-Binding Transcription Factor 3
Otf-3
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Reactome: R-HSA-9754189
Reactome: R-HSA-9823739
Reactome: R-HSA-9827857
Reactome: R-HSA-9834899
Cellular Localisation Cytoplasm
Nucleus
Expressed In A Diffuse And Slightly Punctuate Pattern
Colocalizes With Mapk8 And Mapk9 In The Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 5 - Transcription Factor 1 antibody
Anti-Octamer-Binding Protein 3 antibody
Anti-Oct-3 antibody
Anti-Octamer-Binding Protein 4 antibody
Anti-Oct-4 antibody
Anti-Octamer-Binding Transcription Factor 3 antibody
Anti-Otf-3 antibody
Anti-POU5F1 antibody
Anti-OCT3 antibody
Anti-OCT4 antibody
Anti-OTF3 antibody

Information sourced from Uniprot.org