Anti-OCT-3/4 antibody [C-10] (STJ16101853)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenAmino acids1-134 of Oct-3/4 of human origin
General Information
| Short Description | Mouse monoclonal anti-OCT-3/4 for use in IHC-F and IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/IHC-P |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | C-10 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purification | Purified |
| Dilution Range | IHC-FIHC-P 1:50-1:100 |
| Formulation | Purified antibody in PBS with 0.2 % BSA and 15mM sodium azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
| Immunogen | Amino acids1-134 of Oct-3/4 of human origin |
Additional Info
| Background | Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programs. Two isoforms of Oct-3, termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3. Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Seminoma or embryonal carcinoma. Staining Nuclear |
Information sourced from Uniprot.org