Anti-OCRL antibody (1-220) (STJ11104387)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Inositol Polyphosphate 5-Phosphatase Ocrl (1-220) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OCRL |
| Gene ID | 4952 |
| Uniprot ID | OCRL_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MEPPLPVGAQPLATVEGMEM KGPLREPCALTLAQRNGQYE LIIQLHEKEQHVQDIIPINS HFRCVQEAEETLLIDIASNS GCKIRVQGDWIRERRFEIPD EEHCLKFLSAVLAAQKAQSQ LLVPEQKDSSSWYQKLDTKD KPSVFSGLLGFEDNFSSMNL DKKINSQNQPTGIHREPPPP PFSVNKMLPREKEASNKEQP KVTNTMRKLFVPNTQSGQRE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OCRL (NP_001578.2). |
Additional Info
| Tissue Specificity | Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts. Expressed in the retina and the retinal pigment epithelium. |
| Function | Catalyzes the hydrolysis of the 5-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2. Able also to hydrolyze the 5-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate. Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes. Involved in primary cilia assembly. Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling. |
| Protein Name | Inositol Polyphosphate 5-Phosphatase OcrlInositol Polyphosphate 5-Phosphatase Ocrl-1Ocrl-1Lowe Oculocerebrorenal Syndrome ProteinPhosphatidylinositol 3 -4 -5-Triphosphate 5-Phosphatase |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-1660514Reactome: R-HSA-1855183Reactome: R-HSA-1855204Reactome: R-HSA-432722Reactome: R-HSA-8856828Reactome: R-HSA-9013409Reactome: R-HSA-9013423 |
| Cellular Localisation | Cytoplasmic VesiclePhagosome MembraneEarly Endosome MembraneMembraneClathrin-Coated PitCell ProjectionCiliumPhotoreceptor Outer SegmentEndosomeGolgi ApparatusTrans-Golgi NetworkLysosomeAlso Found On MacropinosomesColocalized With Appl1 On Phagosomes |
| Alternative Antibody Names | Anti-Inositol Polyphosphate 5-Phosphatase Ocrl antibodyAnti-Inositol Polyphosphate 5-Phosphatase Ocrl-1 antibodyAnti-Ocrl-1 antibodyAnti-Lowe Oculocerebrorenal Syndrome Protein antibodyAnti-Phosphatidylinositol 3 -4 -5-Triphosphate 5-Phosphatase antibodyAnti-OCRL antibodyAnti-OCRL1 antibody |
Information sourced from Uniprot.org