• Western blot analysis of lysates from wild type (WT) and OCRL knockout (KO) 293T cells, using [KO Validated] OCRL Rabbit polyclonal antibody (STJ11100852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-OCRL antibody (1-220) (STJ11100852)

SKU:
STJ11100852

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OCRL (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OCRL
Gene ID: 4952
Uniprot ID: OCRL_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OCRL (NP_001578.2).
Immunogen Sequence: MEPPLPVGAQPLATVEGMEM KGPLREPCALTLAQRNGQYE LIIQLHEKEQHVQDIIPINS HFRCVQEAEETLLIDIASNS GCKIRVQGDWIRERRFEIPD EEHCLKFLSAVLAAQKAQSQ LLVPEQKDSSSWYQKLDTKD KPSVFSGLLGFEDNFSSMNL DKKINSQNQPTGIHREPPPP PFSVNKMLPREKEASNKEQP KVTNTMRKLFVPNTQSGQRE
Tissue Specificity Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts. Expressed in the retina and the retinal pigment epithelium.
Function Catalyzes the hydrolysis of the 5-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2. Able also to hydrolyze the 5-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate. Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes. Involved in primary cilia assembly. Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling.
Protein Name Inositol Polyphosphate 5-Phosphatase Ocrl
Inositol Polyphosphate 5-Phosphatase Ocrl-1
Ocrl-1
Lowe Oculocerebrorenal Syndrome Protein
Phosphatidylinositol 3 -4 -5-Triphosphate 5-Phosphatase
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1660514
Reactome: R-HSA-1855183
Reactome: R-HSA-1855204
Reactome: R-HSA-432722
Reactome: R-HSA-8856828
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasmic Vesicle
Phagosome Membrane
Early Endosome Membrane
Membrane
Clathrin-Coated Pit
Cell Projection
Cilium
Photoreceptor Outer Segment
Endosome
Golgi Apparatus
Trans-Golgi Network
Lysosome
Also Found On Macropinosomes
Colocalized With Appl1 On Phagosomes
Alternative Antibody Names Anti-Inositol Polyphosphate 5-Phosphatase Ocrl antibody
Anti-Inositol Polyphosphate 5-Phosphatase Ocrl-1 antibody
Anti-Ocrl-1 antibody
Anti-Lowe Oculocerebrorenal Syndrome Protein antibody
Anti-Phosphatidylinositol 3 -4 -5-Triphosphate 5-Phosphatase antibody
Anti-OCRL antibody
Anti-OCRL1 antibody

Information sourced from Uniprot.org

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