• Western blot analysis of various lysates using NUSAP1 Rabbit polyclonal antibody (STJ118445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NUSAP1 antibody (1-61) (STJ118445)

SKU:
STJ118445

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUSAP1 (1-61) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUSAP1
Gene ID: 51203
Uniprot ID: NUSAP_HUMAN
Immunogen Region: 1-61
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-61 of human NUSAP1 (NP_060924.4).
Immunogen Sequence: MIIPSLEELDSLKYSDLQNL AKSLGLRANLRATKLLKALK GYIKHEARKGNENQDESQTS A
Post Translational Modifications Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein. Phosphorylation by ATM in G2/M-phase induces mitotic arrest.
Function Microtubule-associated protein with the capacity to bundle and stabilize microtubules. May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
Protein Name Nucleolar And Spindle-Associated Protein 1
Nusap
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Cytoskeleton
Spindle
Chromosome
Found In The Cytoplasm And Nucleolus During Interphase And Redistributes To The Mitotic Spindle In Prometaphase
Localizes To The Mitotic Spindle During Anaphase And Telophase Then Disappears From Around The Chromosomes During Cytokinesis
Localizes To Multiple Distinct Regions Of Chromosomes Throughout Mitosis
Alternative Antibody Names Anti-Nucleolar And Spindle-Associated Protein 1 antibody
Anti-Nusap antibody
Anti-NUSAP1 antibody
Anti-ANKT antibody
Anti-BM-037 antibody
Anti-PRO0310 antibody

Information sourced from Uniprot.org

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