| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NUP50 |
| Gene ID: | 10762 |
| Uniprot ID: | NUP50_HUMAN |
| Immunogen Region: | 340-468 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-468 of human NUP50 (NP_009103.2). |
| Immunogen Sequence: | ENDEPPKVVVTEVKEEDAFY SKKCKLFYKKDNEFKEKGIG TLHLKPTANQKTQLLVRADT NLGNILLNVLIPPNMPCTRT GKNNVLIVCVPNPPIDEKNA TMPVTMLIRVKTSEDADELH KILLEKKDA |
| Tissue Specificity | Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. |
| Function | Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. |
| Protein Name | Nuclear Pore Complex Protein Nup50 50 Kda Nucleoporin Nuclear Pore-Associated Protein 60 Kda-Like Nucleoporin Nup50 |
| Database Links | Reactome: R-HSA-1169408 Reactome: R-HSA-159227 Reactome: R-HSA-159230 Reactome: R-HSA-159231 Reactome: R-HSA-159236 Reactome: R-HSA-165054 Reactome: R-HSA-168271 Reactome: R-HSA-168276 Reactome: R-HSA-168325 Reactome: R-HSA-168333 Reactome: R-HSA-170822 Reactome: R-HSA-180746 Reactome: R-HSA-180910 Reactome: R-HSA-191859 Reactome: R-HSA-3108214 Reactome: R-HSA-3232142 Reactome: R-HSA-3301854 Reactome: R-HSA-3371453 Reactome: R-HSA-4085377 Reactome: R-HSA-4551638 Reactome: R-HSA-4570464 Reactome: R-HSA-4615885 Reactome: R-HSA-5578749 Reactome: R-HSA-5619107 Reactome: R-HSA-6784531 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9705671 |
| Cellular Localisation | Nucleus Nuclear Pore Complex Nucleus Membrane Peripheral Membrane Protein Nucleoplasmic Side Localizes To The Nucleoplasmic Fibrils Of The Nuclear Pore Complex Dissociates From The Npc Structure Early During Prophase Of Mitosis Associates With The Newly Formed Nuclear Membrane During Telophase In The Testis The Localization Changes During Germ Cell Differentiation From The Nuclear Surface In Spermatocytes To The Whole Nucleus (Interior) In Spermatids And Back To The Nuclear Surface In Spermatozoa |
| Alternative Antibody Names | Anti-Nuclear Pore Complex Protein Nup50 antibody Anti-50 Kda Nucleoporin antibody Anti-Nuclear Pore-Associated Protein 60 Kda-Like antibody Anti-Nucleoporin Nup50 antibody Anti-NUP50 antibody Anti-NPAP60L antibody Anti-PRO1146 antibody |
Information sourced from Uniprot.org