• Western blot analysis of various lysates using NUP35 Rabbit polyclonal antibody (STJ114635) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NUP35 antibody (1-326) (STJ114635)

SKU:
STJ114635

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUP35 (1-326) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUP35
Gene ID: 129401
Uniprot ID: NUP35_HUMAN
Immunogen Region: 1-326
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human NUP35 (NP_612142.2).
Immunogen Sequence: MAAFAVEPQGPALGSEPMML GSPTSPKPGVNAQFLPGFLM GDLPAPVTPQPRSISGPSVG VMEMRSPLLAGGSPPQPVVP AHKDKSGAPPVRSIYDDISS PGLGSTPLTSRRQPNISVMQ SPLVGVTSTPGTGQSMFSPA SIGQPRKTTLSPAQLDPFYT QGDSLTSEDHLDDSWVTVFG FPQASASYILLQFAQYGNIL KHVMSNTGNWMHIRYQSKLQ ARKALSKDGRIFGESIMIG
Function Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
Protein Name Nucleoporin Nup35
35 Kda Nucleoporin
Mitotic Phosphoprotein 44
Mp-44
Nuclear Pore Complex Protein Nup53
Nucleoporin Nup53
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-6784531
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9615933
Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Nuclear Pore Complex
Nucleus Membrane
Peripheral Membrane Protein
Tightly Associated With The Nuclear Membrane And Lamina
Alternative Antibody Names Anti-Nucleoporin Nup35 antibody
Anti-35 Kda Nucleoporin antibody
Anti-Mitotic Phosphoprotein 44 antibody
Anti-Mp-44 antibody
Anti-Nuclear Pore Complex Protein Nup53 antibody
Anti-Nucleoporin Nup53 antibody
Anti-NUP35 antibody
Anti-MP44 antibody
Anti-NUP53 antibody

Information sourced from Uniprot.org

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