• Western blot analysis of various lysates, using NUP155 Rabbit monoclonal antibody (STJ11104195) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of lysates from Jurkat cells, using NUP155 Rabbit monoclonal antibody (STJ11104195) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NUP155 antibody (901-1050) [S4195RM] (STJ11104195)

SKU:
STJ11104195

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Nuclear Pore Complex Protein Nup155 (901-1050) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4195RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUP155
Gene ID: 9631
Uniprot ID: NU155_HUMAN
Immunogen Region: 901-1050
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 901-1050 of human NUP155 (NP_705618.1).
Immunogen Sequence: LKEYQKISNQVDLSNVCAQY RQVRFYEGVVELSLTAAEKK DPQGLGLHFYKHGEPEEDIV GLQAFQERLNSYKCITDTLQ ELVNQSKAAPQSPSVPKKPG PPVLSSDPNMLSNEEAGHHF EQMLKLSQRSKDELFSIALY NWLIQVDLAD
Tissue Specificity Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Phosphorylated. Phosphorylation and dephosphorylation may be important for the function of NUP155 and may play a role in the reversible disassembly of the nuclear pore complex during mitosis. Disulfide-linked to NUP62. The inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.
Function Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.
Protein Name Nuclear Pore Complex Protein Nup155
155 Kda Nucleoporin
Nucleoporin Nup155
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-6784531
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9615933
Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Nuclear Pore Complex
Nucleus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Nucleoplasmic Side
In Mitosis
Assumes A Diffuse Cytoplasmic Distribution Probably As A Monomer
Before Reversing Back Into A Punctate Nuclear Surface Localization At The End Of Mitosis
Alternative Antibody Names Anti-Nuclear Pore Complex Protein Nup155 antibody
Anti-155 Kda Nucleoporin antibody
Anti-Nucleoporin Nup155 antibody
Anti-NUP155 antibody
Anti-KIAA0791 antibody

Information sourced from Uniprot.org

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