• STJ70112 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70112 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70112 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ70112 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70112 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.

Anti-NUMB antibody (C-Term) (STJ70112)

SKU:
STJ70112-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF/FC
Reactivity: Human/Mouse/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NUMB (C-Term) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-6-8µg/ml
IF-Strong expression of the protein seen in the cell junctions/plasma membrane and cytoplasm of A431 and NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NUMB
Gene ID: 8650
Uniprot ID: NUMB_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001005743.1; 001005744.1; NP_003735; NP_001005745.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet
Immunogen Sequence: PFSSDLQKTFEIEL
Function Regulates clathrin-mediated receptor endocytosis. Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Protein Name Protein Numb Homolog
H-Numb
Protein S171
Database Links Reactome: R-HSA-2122948
Reactome: R-HSA-437239
Reactome: R-HSA-5610780
Reactome: R-HSA-5632684
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endosome Membrane
Localizes To Perinuclear Endosomes In An Aak1-Dependent Manner
Alternative Antibody Names Anti-Protein Numb Homolog antibody
Anti-H-Numb antibody
Anti-Protein S171 antibody
Anti-NUMB antibody
Anti-C14orf41 antibody

Information sourced from Uniprot.org

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