| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-U8 snoRNA-decapping enzyme (2-52) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NUDT16 |
| Gene ID: |
131870 |
| Uniprot ID: |
NUD16_HUMAN |
| Immunogen Region: |
2-52 |
| Specificity: |
This antibody detects endogenous levels of human NUD16 |
| Immunogen: |
Synthesized peptide derived from human NUD16 AA range: 2-52 |
| Function | RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA.metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase.hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Exhibits decapping activity towards NAD-capped RNAs and FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro. |
| Protein Name | U8 Snorna-Decapping EnzymeIdp PhosphataseIdpaseInosine Diphosphate PhosphataseNucleoside Diphosphate-Linked Moiety X Motif 16Nudix Motif 16Nudix Hydrolase 16U8 Snorna-Binding Protein H29kM7gpppn-Mrna Hydrolase |
| Database Links | Reactome: R-HSA-2393930 |
| Cellular Localisation | NucleusNucleoplasmNucleolusCytoplasmLocalized Predominantly In The CytoplasmLocalized In NucleolusAnd In A Minor Proportion In Distinct Foci In The Nucleoplasm |
| Alternative Antibody Names | Anti-U8 Snorna-Decapping Enzyme antibodyAnti-Idp Phosphatase antibodyAnti-Idpase antibodyAnti-Inosine Diphosphate Phosphatase antibodyAnti-Nucleoside Diphosphate-Linked Moiety X Motif 16 antibodyAnti-Nudix Motif 16 antibodyAnti-Nudix Hydrolase 16 antibodyAnti-U8 Snorna-Binding Protein H29k antibodyAnti-M7gpppn-Mrna Hydrolase antibodyAnti-NUDT16 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
