| Host: | Mouse |
| Applications: | ELISA/WB/ICC |
| Reactivity: | Chicken/Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Nucleoporin p62 (N-Term) for use in ELISA, WB and ICC in Chicken, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | M436 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Protein A Purified |
| Dilution Range: | WB 1:500ICC 1:50 |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | NUP62 |
| Gene ID: | 23636 |
| Uniprot ID: | NUP62_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | This antibody detects a 62 kDa* protein corresponding to the apparent molecular mass of nucleoporin p62 on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Nucleoporin p62 specifically stains nuclei and nuclear enve |
| Immunogen: | Clone M436 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human nucleoporin p62. This sequence has high homology to similar regions in rat, mouse, and chicken nucleoporin p62. |
| Immunogen Sequence: | Human |
| Post Translational Modifications | O-glycosylated. Contains about 10 N-acetylglucosamine side chain sites predicted for the entire protein, among which only one in the C-terminal. The inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC. |
| Function | Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown. |
| Protein Name | Nuclear Pore Glycoprotein P6262 Kda NucleoporinNucleoporin Nup62 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-6784531Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9615933Reactome: R-HSA-9705671 |
| Cellular Localisation | NucleusNuclear Pore ComplexCytoplasmCytoskeletonSpindle PoleNucleus EnvelopeMicrotubule Organizing CenterCentrosomeCentral Region Of The Nuclear PoreWithin The TransporterDuring Mitotic Cell DivisionIt Associates With The Poles Of The Mitotic Spindle |
| Alternative Antibody Names | Anti-Nuclear Pore Glycoprotein P62 antibodyAnti-62 Kda Nucleoporin antibodyAnti-Nucleoporin Nup62 antibodyAnti-NUP62 antibody |
Information sourced from Uniprot.org
