| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IF/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Nucleophosmin/NPM1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 37kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6kDa according to Human NP_001341935.1 and Mouse NP_0 |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
NPM1 |
| Gene ID: |
4869 |
| Uniprot ID: |
NPM_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_001341935.1; NP_954654.1; NP_001341936.1; NP_001341939.1 |
| Immunogen Sequence: |
QEAIQDLWQWRKSL |
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1. |
| Protein Name | NucleophosminNpmNucleolar Phosphoprotein B23Nucleolar Protein No38Numatrin |
| Database Links | Reactome: R-HSA-180746Reactome: R-HSA-3899300Reactome: R-HSA-606279Reactome: R-HSA-6804115Reactome: R-HSA-8869496Reactome: R-HSA-9692914Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371Reactome: R-HSA-9833482 |
| Cellular Localisation | NucleusNucleolusNucleoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeGenerally NucleolarBut Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer DrugsHas Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml)But Not With Secondary AmlCo-Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The NucleolusColocalized With Nucleolin And Apex1 In NucleoliIsoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During MitosisCan Shuttle Between Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Nucleophosmin antibodyAnti-Npm antibodyAnti-Nucleolar Phosphoprotein B23 antibodyAnti-Nucleolar Protein No38 antibodyAnti-Numatrin antibodyAnti-NPM1 antibodyAnti-NPM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
