• Western blot analysis of extracts of Mouse lung, using NUCKS1 antibody (STJ11104177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using NUCKS1 antibody (STJ11104177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NUCKS1 antibody (100-200) (STJ11104177)

SKU:
STJ11104177

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nuclear Ubiquitous Casein And Cyclin-Dependent Kinase Substrate 1 (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUCKS1
Gene ID: 64710
Uniprot ID: NUCKS_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human NUCKS1 (NP_073568.2).
Immunogen Sequence: ASKQREMLMEDVGSEEEQEE EDEAPFQEKDSGSDEDFLME DDDDSDYGSSKKKNKKMVKK SKPERKEKKMPKPRLKATVT PSPVKGKGKVGRPTASKASK E
Tissue Specificity Widely expressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung.
Post Translational Modifications Phosphorylated in an ATM-dependent manner in response to DNA damage. Phosphorylated by CDK1 and casein kinase.
Function Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, but with less affinity than RAD51AP1.
Protein Name Nuclear Ubiquitous Casein And Cyclin-Dependent Kinase Substrate 1
P1
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Nuclear Ubiquitous Casein And Cyclin-Dependent Kinase Substrate 1 antibody
Anti-P1 antibody
Anti-NUCKS1 antibody
Anti-NUCKS antibody
Anti-JC7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance