| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NUAK1 (362-661) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NUAK1 |
| Gene ID: |
9891 |
| Uniprot ID: |
NUAK1_HUMAN |
| Immunogen Region: |
362-661 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 362-661 of human NUAK1 (NP_055655.1). |
| Immunogen Sequence: |
LERQRSLKKSKKENDFAQSG QDAVPESPSKLSSKRPKGIL KKRSNSEHRSHSTGFIEGVV GPALPSTFKMEQDLCRTGVL LPSSPEAEVPGKLSPKQSAT MPKKGILKKTQQRESGYYSS PERSESSELLDSNDVMGSSI PSPSPPDPARVTSHSLSCRR KGILKHSSKYSAGTMDPALV SPEMPTLESLSEPGVPAEGL SRSYSRPSSVISDDSVLSSD SFDLLDLQENRPARQRIRS |
| Tissue Specificity | Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines. |
| Post Translational Modifications | Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X. Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not dephosphorylated by the myosin PP1 complex when regulating its activity, due to the presence of PPP1R12A, which prevents myosin PP1 from dephosphorylating NUAK1. Phosphorylated by STK38L upon stimulation with IGF1. |
| Function | Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair. |
| Protein Name | Nuak Family Snf1-Like Kinase 1Ampk-Related Protein Kinase 5Ark5Omphalocele Kinase 1 |
| Database Links | Reactome: R-HSA-6804756 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Nuak Family Snf1-Like Kinase 1 antibodyAnti-Ampk-Related Protein Kinase 5 antibodyAnti-Ark5 antibodyAnti-Omphalocele Kinase 1 antibodyAnti-NUAK1 antibodyAnti-ARK5 antibodyAnti-KIAA0537 antibodyAnti-OMPHK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
