• Immunofluorescence analysis of paraffin-embedded Rat small intestine using NTS Rabbit polyclonal antibody (STJ114208) at dilution of 1:350. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NTS Rabbit polyclonal antibody (STJ114208) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NTS antibody (24-148) (STJ114208)

SKU:
STJ114208

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NTS (24-148) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NTS
Gene ID: 4922
Uniprot ID: NEUT_HUMAN
Immunogen Region: 24-148
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-148 of human NTS (NP_006174.1).
Immunogen Sequence: SDSEEEMKALEADFLTNMHT SKISKAHVPSWKMTLLNVCS LVNNLNSPAEETGEVHEEEL VARRKLPTALDGFSLEAMLT IYQLHKICHSRAFQHWELIQ EDILDTGNDKNGKEEVIKRK IPYIL
Post Translational Modifications Neurotensin: Neurotensin is cleaved and degraded by Angiotensin-converting enzyme (ACE) and neprilysin (MME).
Function Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.
Protein Name Neurotensin/Neuromedin N Cleaved Into - Large Neuromedin N
Nmn-125 - Neuromedin N
Nn
Nmn - Neurotensin
Nt - Tail Peptide
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Packaged Within Secretory Vesicles
Alternative Antibody Names Anti-Neurotensin/Neuromedin N Cleaved Into - Large Neuromedin N antibody
Anti-Nmn-125 - Neuromedin N antibody
Anti-Nn antibody
Anti-Nmn - Neurotensin antibody
Anti-Nt - Tail Peptide antibody
Anti-NTS antibody

Information sourced from Uniprot.org

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