• Western blot analysis of extracts of various cell lines, using CD73/NT5E rabbit polyclonal antibody (STJ11102804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using CD73/NT5E rabbit polyclonal antibody (STJ11102804) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using CD73/NT5E rabbit polyclonal antibody (STJ11102804) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using CD73/NT5E rabbit polyclonal antibody (STJ11102804) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NT5E antibody (32-264) (STJ11102804)

SKU:
STJ11102804

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NT5E (32-264) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NT5E
Gene ID: 4907
Uniprot ID: 5NTD_HUMAN
Immunogen Region: 32-264
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-264 of human CD73/NT5E (NP_002517.1).
Immunogen Sequence: LHTNDVHSRLEQTSEDSSKC VNASRCMGGVARLFTKVQQI RRAEPNVLLLDAGDQYQGTI WFTVYKGAEVAHFMNALRYD AMALGNHEFDNGVEGLIEPL LKEAKFPILSANIKAKGPLA SQISGLYLPYKVLPVGDEVV GIVGYTSKETPFLSNPGTNL VFEDEITALQPEVDKLKTLN VNKIIALGHSGFEMDKLIAQ KVRGVDVVVGGHSNTFLYTG NPPSKEVPAGKYP
Function Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.
Protein Name 5'-Nucleotidase
5'-Nt
5'-Deoxynucleotidase
Ecto-5'-Nucleotidase
Imp-Specific 5'-Nucleotidase
Thymidylate 5'-Phosphatase
Cd Antigen Cd73
Database Links Reactome: R-HSA-196807
Reactome: R-HSA-73621
Reactome: R-HSA-74259
Reactome: R-HSA-9660826
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-5'-Nucleotidase antibody
Anti-5'-Nt antibody
Anti-5'-Deoxynucleotidase antibody
Anti-Ecto-5'-Nucleotidase antibody
Anti-Imp-Specific 5'-Nucleotidase antibody
Anti-Thymidylate 5'-Phosphatase antibody
Anti-Cd Antigen Cd73 antibody
Anti-NT5E antibody
Anti-NT5 antibody
Anti-NTE antibody

Information sourced from Uniprot.org

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