• Western blot analysis of A-549, using Neuropilin-1 (NRP1) antibody (STJ11104753) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:200000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-NRP1 antibody (22-644) [S4753RM] (STJ11104753)

SKU:
STJ11104753

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Neuropilin-1 (22-644) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4753RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRP1
Gene ID: 8829
Uniprot ID: NRP1_HUMAN
Immunogen Region: 22-644
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-644 of human Neuropilin-1 (NRP1) (NP_003864.5).
Immunogen Sequence: FRNDKCGDTIKIESPGYLTS PGYPHSYHPSEKCEWLIQAP DPYQRIMINFNPHFDLEDRD CKYDYVEVFDGENENGHFRG KFCGKIAPPPVVSSGPFLFI KFVSDYETHGAGFSIRYEIF KRGPECSQNYTTPSGVIKSP GFPEKYPNSLECTYIVFVPK MSEIILEFESFDLEPDSNPP GGMFCRYDRLEIWDGFPDVG PHIGRYCGQKTPGRIRSSSG ILSMVFYTDSAIAKEGFSA
Tissue Specificity Isoform 1: The expression of isoforms 1 and 2 does not seem to overlap. Expressed in olfactory epithelium (at protein level). Expressed in fibroblasts (at protein level). Expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta.moderately in lung, liver, skeletal muscle, kidney and pancreas.and low in adult brain. Expressed in the central nervous system, including olfactory related regions such as the olfactory tubercles and paraolfactory gyri. Isoform 2: The expression of isoforms 1 and 2 does not seem to overlap. Found in liver hepatocytes, kidney distal and proximal tubules.
Function Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection. Isoform 2: Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Protein Name Neuropilin-1
Vascular Endothelial Cell Growth Factor 165 Receptor
Cd Antigen Cd304
Database Links Reactome: R-HSA-194306
Reactome: R-HSA-376176
Reactome: R-HSA-399954
Reactome: R-HSA-399955
Reactome: R-HSA-399956
Reactome: R-HSA-445144
Reactome: R-HSA-447041
Reactome: R-HSA-9694614
Cellular Localisation Isoform 2: Secreted
Mitochondrion Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Cytoplasm
Alternative Antibody Names Anti-Neuropilin-1 antibody
Anti-Vascular Endothelial Cell Growth Factor 165 Receptor antibody
Anti-Cd Antigen Cd304 antibody
Anti-NRP1 antibody
Anti-NRP antibody
Anti-VEGF165R antibody

Information sourced from Uniprot.org

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