| Host: |
Mouse |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-NR5A1 (220-461aa) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
N1665 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1/Kappa |
| Formulation: |
Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: |
Affinity purified |
| Dilution Range: |
1:50‐100 |
| Storage Instruction: |
Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Gene Symbol: |
NR5A1 |
| Gene ID: |
2516 |
| Uniprot ID: |
STF1_HUMAN |
| Immunogen Region: |
220-461aa |
| Specificity: |
Positive control: Adrenal cortical carcinoma |
| Immunogen: |
Synthesized peptide corresponding to amino acids 220-461 SF-1 protein |
| Tissue Specificity | High expressed in the adrenal cortex, the ovary, the testis, and the spleen. |
| Post Translational Modifications | Acetylation stimulates the transcriptional activity. Sumoylation reduces CDK7-mediated phosphorylation on Ser-203. Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity. |
| Function | Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. |
| Protein Name | Steroidogenic Factor 1Sf-1Stf-1Hsf-1Adrenal 4-Binding ProteinFushi Tarazu Factor Homolog 1Nuclear Receptor Subfamily 5 Group A Member 1Steroid Hormone Receptor Ad4bp |
| Database Links | Reactome: R-HSA-383280Reactome: R-HSA-4090294Reactome: R-HSA-452723Reactome: R-HSA-9690406 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Steroidogenic Factor 1 antibodyAnti-Sf-1 antibodyAnti-Stf-1 antibodyAnti-Hsf-1 antibodyAnti-Adrenal 4-Binding Protein antibodyAnti-Fushi Tarazu Factor Homolog 1 antibodyAnti-Nuclear Receptor Subfamily 5 Group A Member 1 antibodyAnti-Steroid Hormone Receptor Ad4bp antibodyAnti-NR5A1 antibodyAnti-AD4BP antibodyAnti-FTZF1 antibodyAnti-SF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
